Effect of mangiferin on the proliferation of rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2012.49.023

Abstract

Abstract:

BACKGROUND: Tissue engineering requires a lot of seed cells, and the reports on mangiferin promotion of bone mesenchymal stem cells proliferation are rare.
OBJECTIVE: To observe the effects of mangiferin on the proliferation of rat bone marrow mesenchymal stem cells in vitro.
METHODS: Bone marrow mesenchymal stem cells from rats were isolated, cultured and purified by the whole bone marrow adherence method. The MTT method was used to detect the cytotoxicity of mangiferin and the growth curve was drawn, and the cell cycle detection and the cell proliferation tracer detection were used to observe the effect of mangiferin at different concentrations on the biological characteristics of rat bone marrow mesenchymal stem cells, as well as the proliferation of bone marrow mesenchymal stem cells.
RESULTS AND CONCLUSION: In a certain range of concentration, different concentrations of mangiferin could promote the proliferation and differentiation of bone marrow mesenchymal stem cells, and showed a dose and time dependent manner. The mangiferin at the concentration of 20 μmol/L and 40 μmol/L had the most significant effect (P < 0.01); followed by the concentration of 80 μmol/L induction for 48 and 72 hours (P < 0.05). The S phase ratio of the cells induced with 20, 40 and 80 μmol/L mangiferin was higher than that of the normal cells, and the S phase ratio of the bone marrow mesenchymal stem cells induced with 40 μmol/L mangiferin was the highest (P < 0.05). Mangiferin capromote the proliferation of bone marrow mesenchymal stem cells in vitro.Li XF, Zhao JM, Luo SX, Cheng JW, Liu JT. Effect of mangiferin on the proliferation of rat bone marrow mesenchymal stem cells.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(49):9247-9252.

CLC Number:

R394.2

Li Xiao-feng, Zhao Jin-min, Luo Shi-xing, Cheng Jian-wen, Liu Jun-ting. Effect of mangiferin on the proliferation of rat bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9247-9252.

Figures/Tables 8

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